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Experimental Non-obstructive Chronic Renal Infection Model with Planktonic and Biofilm Cells of Pseudomonas Aeruginosa

¹Department of Microbiology, Panjab University, Chandigarh, INDIA.

²Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California, USA

³Department of Pathology, PGIMER, Chandigarh, INDIA.

*Corresponding Author:

Dr Rahul Mittal

Division of Infectious Diseases, MS#51

Childrens Hospital Los Angeles,

4650 Sunset Boulevard

Los Angeles, CA, 90027, USA

Telephone:  323-361-5809

E-Mail: ramittal@chla.usc.edu; rahul_mittal20022@yahoo.com

Abstract

Availability of an acceptable animal model, which closely mimics human clinical situations in terms of receptors and route of entry, is of utmost importance to study host parasite interaction. In the present study, Swiss Webster (LACA) strain of female mice was chosen for induction of chronic renal inflammation by planktonic as well as biofilm cells of Pseudomonas aeruginosa. Bacterial inoculum was instilled thrice intrauretherally with the help of catheter. Both the cell forms were able to induce chronic renal inflammation in the experimental animals. However, severity of lesions induced by four day old biofilm cells was more as compared to planktonic cells. The chronic pyelonephritis model holds great promise for understanding the biology and pathogenesis of P. aeruginosa induced urinary tract infections as well as in development of effective preventive strategies.

Keywords: Pseudomonas aeruginosa; Planktonic cells; Biofilm cells; Chronic renal infection; UTI mouse model.

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