| Am. J. Biomed. Sci. 2009, 1(2), 126-132; doi: 10.5099/aj090200126 |
Agglutination of Human Spermatozoa Due to Human Semen Culture Bacterial Isolates Bearing Sperm Ligand |
|
Vijay Prabha, Neha Thakur, Siftjit Kaur, Navchetan Kaur, Arvind Singh and Sushila Kala |
|
Department of Microbiology, Panjab University, Chandigarh- 160014, India. |
|
*Corresponding Author: |
|
Dr. Vijay Prabha |
|
|
|
|
|
|
|
|
|
|
|
E mail: satishvijay11@yahoo.com |
|
Abstract Sperm ligand on Escherichia coli that agglutinates human spermatozoa has been
extracted from washed E. coli cells
by incubating with 3M NaCl for 12 h at 37ºC under shake conditions (150 rpm).
Further the sperm ligand was purified after extensive dialysis against double
distilled water by step down chromatography procedures (molecular weight
sieving, anion exchange). The purification procedure yielded a protein of
apparent homogeneity. The ligand was heat labile as it could not withstand a
temperature of 60ºC for 10 minutes. The ligand did not pass through the
dialysis tubing. The molecular weight was found to be ~71KDa. Electron microscopy
studies of washed sperm cells treated with purified ligand showed profound
morphological alterations to head, neck and tail. Keywords: E.
coli; ligand; spermatozoa; agglutination; scanning electron microscopy. Download the full article (PDF)
|
© American Journal of Biomedical Sciences 2007.
All Rights Reserved.