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An Amperometric Nitrite Biosensor Based on the Bioelectrocatalysis of Hemoglobin Incorporated in Sol-gel Film

^a School of Sciences, Zhejiang Forestry University, Lin'an 311300. P R China

^b Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, P. R. China

*Corresponding Author:

S. Wang

Phone: +86-27-50865309

Fax: +86-27-88663043

E-mail address: wangsf@hubu.edu.cn (S. Wang)

Abstract

A highly sensitive, fast and stable biosensor for determination of nitrite was developed using hemoglobin immobilized on a poly (N-isopropylacyamide-co-3- methacryloxypropyltrimethoxysilane) (PNM) modified glass carbon electrodes. The matrix provided a biocompatible microenvironment for retaining the native structure and activity of the entrapped hemoglobin. Nitrite could be reduced by the electrocatalysis of the entrapped hemoglobin without any mediator. The reagentless sensor exhibited a fast response (less than 8 s) and sensitivity as high as 0.73 μA mM cm^-2. The linear range for nitrite determination was from 0.11 to 1.88 mM with a detection limit of 2.0x10^-5 mol L^-1.

Keywords:  Hemoglobin; PNM; Bioelectrocatalysis; Biosensor; Nitrite.

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