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Flow-injection Post-chemiluminescence Determination of Furazolidone in Animal Feeds

College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR. China

*Corresponding Author:

Dr. Heyou Han, professor

College of Science

State Key Laboratory of Agricultural Microbiology

Huazhong Agricultural University

Wuhan 430070, PR. China

Tel: +86-27-87288246;

E-mail: hyhan@mail.hzau.edu.cn

Abstract

The post-chemiluminescence (PCL) reaction was observed with the mixture solution after completion CL reaction of luminol-H₂O₂ in present of Furazolidone (FZD). A novel method for the determination of FZD was established by flow-injection PCL. The CL intensity responded linearly to the concentration of FZD in the range 1.0x10⁻⁷-1.0x10^{−5 g} mL^-1, with a linear correlation of 0.9949. The detection limit for FZD was 1.96x10^{−8 g} mL^-1 (S/N=3). The relative standard deviation (RSD) was 3.5% (n=11, c=1.0x10^{−6 g} mL^-1). The proposed method has been applied to the determination of FZD in animal feeds and the results were consistent with those obtained by the HPLC-UV method. A possible mechanism for the PCL reaction was also discussed by the investigation of CL kinetic characteristics, CL spectra and UV-Vis absorption spectra.

Keywords:  Post chemiluminescence; furazolidone; luminol.

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