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A Cell-based Drug Efflux Assay for Analysis of Multidrug Resistance in Cancer Patients

¹Department of Biopharma Sciences, Chandigarh Group of Colleges, Gharuan, Mohali (Punjab), India.

²Department of Biochemistry, Gian Sagar Medical College and Hospital, Banur, Patiala (Punjab), India.

*Corresponding author

Dr. Arvinder Singh

Assistant Professor (HOD),

Department of Biopharma Sciences, Chandigarh Group of Colleges,

Gharuan, Mohali (Punjab), India.

Tel: +919878281115

Email: arvin111@gmail.com

Abstract

Multidrug Resistance (MDR) is a phenomenon where many tumor cells selected for resistance to a single agent are cross-resistant to several structurally and functionally unrelated compounds. Polymorphisms of genes encoding for xenobiotics and drug transporters are potential factors, which can influence the risk of developing cancer and its clinical outcome. The single-nucleotide polymorphism C3435T of MDR1 gene has been found to be associated with altered tissue expression and function of Permeability-glycoprotein (PGP). In the present study efflux of drug amongst different genotypes was studied using cell based assay. Drug uptake of the cells was found to be maximum in the cells that showed homozygous TT genotype in contrast to the cells with homozygous CC genotype that showed minimum drug uptake. This method is a novel approach for the study of such kind of pharmacokinetics. The results of the present study may prove very helpful in the treatment of cancer patients. 

Keywords:  ATP-binding Cassette; Multidrug resistance; Permeability-glycoprotein; Single nucleotide polymorphism.

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