Stabilizing Ability and Anti-sickling Potentials of Ganoderma Lucidum Decoction Extract on Human HbS Erythrocytes Membrane

Department of Biochemistry, Faculty of science, University of Port Harcourt. P. M. B. 5323, East-West Road, Choba, Rivers State, Nigeria.

^*Corresponding Author

Reginald Chibueze Ohiri

Department of Biochemistry, Faculty of science

University of Port Harcourt. P. M. B. 5323, East-West Road, Choba

Rivers State

Nigeria

Email: raycohiri@yahoo.com, reginald.ohiri@uniport.edu.ng

Tel:+234 8038808077

Abstract

Anti-sickling properties of Ganoderma lucidum decoction extract were studied using human HbS erythrocytes. The % lysis, median corpuscular fragility, % stability, polymerisation rate and morphological deformations were determined. The lowest % lysis of (6.76 + 1.63% and 6.15 + 1.96%) were observed in cells treated with 5 mg/ml of extract at 7.0 g/l and 9.0 g/l saline concentrations, while increase in median corpuscular fragility of the erythrocytes were observed, with values of 5.33 + 0.41 g/100g, 5.99 + 0.83 g/100g and 6.08 + 0.51 g/100g for 5 mg/ml, 10 mg/ml and 15 mg/ml of extract. Erythrocytes percentage stability also increased with values of 12.92 + 0.86 %, 26.91 + 1.05 % and 28.81 + 0.94 % for 5 mg/ml, 10 mg/ml and 15 mg/ml of extract, while a pronounced decrease in haemoglobin polymerisation rate was observed at 15 mg/ml of extract from the 2nd to the 3rd minute of analysis with values 0.78 + 0.12 %, 0.47 + 0.07 % and 0.58 + 0.09 % for 2.0, 2.5 and 3.0 minutes. The lowest erythrocytes morphological deformity score was observed at 15.0 mg/ml extract with values of 80.00 + 3.70 %, 56.00 + 2.80 % and 44.00 + 2.20 % at 0.0, 5.0 and 10.0 minutes respectively.

Keywords:Ganoderma lucidum, Decoction extract, Erythrocytes, Stability, Polymerization

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