Role of Single Nucleotide Polymorphism of Matrix Metalloproteinase (MMP-13 rs2252070) in the Etiology of Dental Caries: A PCR Based Study  

¹ Department of Conservative Dentistry and Endodontics, Kathmandu Medical College, Kathmandu, Nepal

² Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, California, USA

³ Department of Conservative Dentistry and Endodontics, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

⁴ Tooth works, Toronto, Ontario, Canada

⁵ Department of Biochemistry, B.P. Koirala Institute of Health Sciences, Dharan, Nepal

^*Corresponding Author

Deepak Kumar Roy

Department of Conservative Dentistry and Endodontics

Kathmandu Medical College, Kathmandu

Nepal

Email: drdeepak48@gmail.com

Abstract

Title: Role Of Single Nucleotide Polymorphism Of Matrix Metalloproteinase (MMP-13 rs2252070) In The Etiology Of Dental Caries: A PCR Based Study.

Background: Dental caries is multifactorial in origin. This study provides an alternative hypothesis for causation of dental caries and emphasize that genetics has a major role in disease process.

Methods: The subjects were divided into 4 groups based on oral hygiene and dental caries experience. Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was used in this study. Blood samples (2 ml) were obtained from the patients followed by isolation of Genomic DNA, Polymerase chain reaction test and digestion with enzyme Bsr-I.

Results: The size of the initial PCR products was 450bp. After digestion with restriction enzyme Bsr-I the 450bp products were completely digested with two restriction sites (250 &180) in a group of subjects with good oral hygiene and presence of dental caries. Other groups had incomplete digestion With Bsr-I.

Conclusion: Due to the single nucleotide polymorphism of MMP-13(rs2252070) the patients were suffering from dental caries despite having good oral hygiene. Genes play a major role in progression of dental caries.

Practical implication: The established relation will help in formulating guidelines for prevention and treatment of cariogenic subjects more effectively. The pattern of dental caries can be learned in more precise manner.

Keywords: Dental caries, Matrixmetalloprteinase-13, Single nucleotide polymorphism

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